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uea 1 rhodamine  (Vector Laboratories)


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    Structured Review

    Vector Laboratories uea 1 rhodamine
    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
    Uea 1 Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/uea+1+rhodamine/Rhodamine+labeled+Ulex+Europaeus+Agglutinin+I+(UEA+I)/bio_rxiv__64898__2026__04__15__718752-82-23-26
    Average 94 stars, based on 190 article reviews
    uea 1 rhodamine - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa"

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa

    Journal: bioRxiv

    doi: 10.64898/2026.04.15.718752

    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
    Figure Legend Snippet: ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Techniques Used: Staining

    Related Articles

    Immunopeptidomics:

    Article Title: Human gut M cells resemble dendritic cells and present gluten antigen.
    Article Snippet: Microfold (M) cells are rare intestinal epithelial cells that reside in the follicle-associated epithelium of Peyer’s patches.. M cells transport luminal antigens to submucosal antigen-presenting cells.. These insights primarily derive from transmission electron microscopy and studies using genetically modified mice.

    Article Title: Human gut M cells resemble dendritic cells and present gluten antigen
    Article Snippet: Leica LAS X software (v.3.5.7.23225) was used for image processing and export. .. Primary antibodies used in this study: mouse anti-human GP2 (MBL, D277-3, clone 3G7-H9, diluted 1:200); mouse anti-human ICAM2 (Thermo Fisher, BMS109BT, clone CBR-IC2/2, diluted 1:200); mouse anti-human HLA-DP/DQ/DR (Thermo Fisher, MA1-25914, clone CR3/43, diluted 1:200); rat anti-mouse MHC-II (I-A/I-E) (Thermo Fisher, 14-5321-82, clone M5/114.15.2, diluted 1:200), UEA-1-Rhodamine (Vector, RL-1062-2, diluted 1:1,000); Phalloidin (Sigma, 65906-10NMOL, diluted 1:1,000); DONQ52 antibody (provided by Chugai Pharmaceutical, diluted 1:100). .. IHC staining images using TFF2 and MHC-II antibodies in primary human intestinal tissues were obtained from the Human Protein Atlas ( https://proteinatlas.org ), details regarding antibodies and patient numbers are included in the related figures.

    Staining:

    Article Title: A nanobody-based tracer targeting DPP6 for non-invasive imaging of human pancreatic endocrine cells.
    Article Snippet: For this purpose, 105 cells from crude digested human pancreata were processed and labelled as in17,49, with TSQ (50 μg/ml; #ENZ-52153, Enzo Life Sciences) that selectively binds islet cell granules. .. In parallel, the exocrine material (acinar and ductal cells) was labelled with UEA-1-Rhodamine (1:2000; #RL-1062, Vector Laboratories) to mark acinar cells; to stain the ductal cells an mAb for CA19-9 (1:100; #15146, Abcam) was used, with subsequent secondary anti-mouse pAb Rhodamine Red X (1:500; #R6393, ThermoFisher). ..

    Article Title: ATG16L1 protects from interferon-γ-induced cell death in the small intestinal crypt.
    Article Snippet: Re Pu The breakdown of the intestinal mucosal barrier is thought to underlie the progression to Crohn disease (CD), whereby numerous risk factors contribute.. For example, a genetic polymorphism of the autophagy gene ATG16L1, associated with an increased risk of developing CD, contributes to the perturbation of the intestinal epithelium.. We examined the role of Atg16l1 in protecting the murine small intestinal epithelium from T-cell-mediated damage using the anti-CD3 model of enteropathy.

    Article Title: A nanobody-based tracer targeting DPP6 for non-invasive imaging of human pancreatic endocrine cells
    Article Snippet: For this purpose, 10 5 cells from crude digested human pancreata were processed and labelled as in , , with TSQ (50 μg/ml; #ENZ-52153, Enzo Life Sciences) that selectively binds islet cell granules. .. In parallel, the exocrine material (acinar and ductal cells) was labelled with UEA-1-Rhodamine (1:2000; #RL-1062, Vector Laboratories) to mark acinar cells; to stain the ductal cells an mAb for CA19-9 (1:100; #15146, Abcam) was used, with subsequent secondary anti-mouse pAb Rhodamine Red X (1:500; #R6393, ThermoFisher). .. The FACS gating strategy to separate endocrine and exocrine pancreatic tissues is shown in Fig. . Acquisition was performed on a Fortezza or on a CantoII (BD biosciences) flow cytometers.

    Incubation:

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa
    Article Snippet: For Lectin staining, slides were deparaffinized in xylene, rehydrated through a graded ethanol series, and blocked for endogenous biotin/streptavidin binding using a Streptavidin/Biotin blocking kit (Vector Laboratories). .. Sections were incubated with biotinylated MAL-II (B-1265-1, Vector Laboratories; 3μg/mL in 1%BSA in PBS) overnight at 4°C, washed in PBS, then incubated with UEA-1 rhodamine (RL-1062-2, Vector Laboratories; 2μg/mL) and streptavidin-FITC (405201, BioLegend; 5μg/mL) in 1% BSA in PBS for 1 hr at room temperature in the dark. ..



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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    Image Search Results


    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Journal: bioRxiv

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa

    doi: 10.64898/2026.04.15.718752

    Figure Lengend Snippet: ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Article Snippet: Sections were incubated with biotinylated MAL-II (B-1265-1, Vector Laboratories; 3μg/mL in 1%BSA in PBS) overnight at 4°C, washed in PBS, then incubated with UEA-1 rhodamine (RL-1062-2, Vector Laboratories; 2μg/mL) and streptavidin-FITC (405201, BioLegend; 5μg/mL) in 1% BSA in PBS for 1 hr at room temperature in the dark.

    Techniques: Staining

    Journal: Acta Histochemica et Cytochemica

    Article Title: Alterations in Ileal Secretory Cells of The DSS-Induced Colitis Model Mice

    doi: 10.1267/ahc.24-00049

    Figure Lengend Snippet: Antibodies and lectins

    Article Snippet: Ulex Europaeus Agglutinin 1 (UEA 1) Rhodamine , Specifically recognizes α-1,2-fucose , Vector Laboratories (Burlingame, CA, USA) , 1:300 , .

    Techniques: Plasmid Preparation

    A ) Merged image of WGA-Alexa fluor 350 (blue), UEA 1-rhodamine (magenta), and phase-contrast image. Bars = 50 µm. B ) High-magnification images of lectin histochemistry of WGA-Alexa 350 conjugate or UEA 1-rhodamine. Bars = 10 µm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Alterations in Ileal Secretory Cells of The DSS-Induced Colitis Model Mice

    doi: 10.1267/ahc.24-00049

    Figure Lengend Snippet: A ) Merged image of WGA-Alexa fluor 350 (blue), UEA 1-rhodamine (magenta), and phase-contrast image. Bars = 50 µm. B ) High-magnification images of lectin histochemistry of WGA-Alexa 350 conjugate or UEA 1-rhodamine. Bars = 10 µm.

    Article Snippet: Ulex Europaeus Agglutinin 1 (UEA 1) Rhodamine , Specifically recognizes α-1,2-fucose , Vector Laboratories (Burlingame, CA, USA) , 1:300 , .

    Techniques: